Scatchard Plot and Heterogeneity in Binding Affinity of Labeled and Unlabeled Ligand
1975; American Association for Clinical Chemistry; Volume: 21; Issue: 12 Linguagem: Inglês
10.1093/clinchem/21.12.1769
ISSN1530-8561
AutoresH.J.G. Hollemans, Rogier M. Bertina,
Tópico(s)Electrochemical Analysis and Applications
ResumoIn saturation analysis the Scatchard plot is a generally accepted method for calculation of the affinity constant, K, and the molar concentration, q, of the binder. However, in a system where the K's for the labeled and unlabeled ligand are unequal, a nonlinear plot can be obtained from which incorrect values for K and q may be calculated. This paper mathematically explains how the plot may deviate and under which conditions there will be a maximum in the curve. When the binding sites are homogeneous, the coordinates of this maximum can be used to calculate K and q. A general mathematical expression is derived on the basis of which a linear curve can be constructed for calculation of q and K, which is valid even when affinity for the labeled and unlabeled ligand is not identical.
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