Portal de Periódicos da CAPES

Establishment of human malignant mesothelioma cell lines

1989; Wiley; Volume: 44; Issue: 2 Linguagem: Inglês

10.1002/ijc.2910440212

1097-0215

Marjan A. Versnel, M.J. Bouts, Henk C. Hoogsteden, Theodorus H. Der Van Kwast, Mick Delahaye, Anne Hagemeijer,

Cellular Mechanics and Interactions

Seventeen human malignant mesothelioma cell lines were isolated from 61 samples (46 effusions, 9 biopsies and 6 tumors obtained at autopsy) collected from patients with a confirmed malignant mesothelioma. The method used is given in detail. Cytogenetic analysis of growing cultures is the best indicator to determine whether the observed proliferation concerns malignant or normal mesothelial cells. The addition of epidermal growth factor (EGF) and hydrocortisone (HC), or EGF alone, to the culture medium increases the chances of successful isolation of a malignant mesothelioma cell line.