Portal de Periódicos da CAPES

In vivo role of ER-associated peptidase activity in tailoring peptides for presentation by MHC class Ia and class Ib molecules

2006; Rockefeller University Press; Volume: 203; Issue: 3 Linguagem: Inglês

10.1084/jem.20052271

1540-9538

Jingbo Yan, Vrajesh V. Parekh, Yanice Mendez-Fernandez, Danyvid Olivares‐Villagómez, Srdjan M. Dragovic, Timothy Hill, Derry C. Roopenian, Sebastian Joyce, Luc Van Kaer,

Immunotherapy and Immune Responses

Endoplasmic reticulum (ER)-associated aminopeptidase (ERAP)1 has been implicated in the final proteolytic processing of peptides presented by major histocompatibility complex (MHC) class I molecules. To evaluate the in vivo role of ERAP1, we have generated ERAP1-deficient mice. Cell surface expression of the class Ia molecules H-2Kb and H-2Db and of the class Ib molecule Qa-2 was significantly reduced in these animals. Although cells from mutant animals exhibited reduced capacity to present several self- and foreign antigens to Kb-, Db-, or Qa-1b–restricted CD8+ cytotoxic T cells, presentation of some antigens was unaffected or significantly enhanced. Consistent with these findings, mice generated defective CD8+ T cell responses against class I–presented antigens. These findings reveal an important in vivo role of ER-associated peptidase activity in tailoring peptides for presentation by MHC class Ia and class Ib molecules.