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Genetic diversity of male fig (Ficus carica caprificus L.) genotypes with random amplified polymorphic DNA (RAPD) markers

2011; Academic Journals; Volume: 10; Issue: 4 Linguagem: Inglês

10.5897/ajb10.995

1684-5315

Zeynel Dalkılıç, H. Osman Mestav, Gonca Guuml, nver-Dalkılıccedil, Hilmi Kocataş,

Plant Pathogenic Bacteria Studies

The objective of this research is to characterize and relate 43 different male fig (Ficus carica caprificus L.) genotypes grown in AydA±n Province, Turkey using random amplified polymorphic DNA (RAPD) markers. A total of 85 10-mer primers were tested with the RAPD technique. A total of 76 polymorphic bands out of 272 bands (27.9%) were observed from 36 primers. The molecular size varied from 200 - 3000 bp. Genotypes were placed in nine different groups in the dendrogram drawn from the similarity matrix. The closest genotypes are ‘Yanako1’ and ‘Yanako2’. The relationship between the genotypes and their respective regions supported by the dendrogram is incongruent. Polymorphic information content (PIC) values ranged from 0.16 (OPD08) to 0.50 (OPC01 and OPC04) for the polymorphic data set. The larger source of variation was observed within populations (88.58%) followed by among populations (11.42%) in the analysis of molecular variance. The relationships among genotypes were defined by the first three principle components (PC), accounting for 40.91% of the total variation at the molecular level. Key words: Ficus carica caprificus, genetic diversity, AydA±n province, random amplified polymorphic DNA markers, fingerprinting, analysis of molecular variance,polymorphic information content, principle component analysis.