Cationic microparticles: A potent delivery system for DNA vaccines
2000; National Academy of Sciences; Volume: 97; Issue: 2 Linguagem: Inglês
10.1073/pnas.97.2.811
ISSN1091-6490
AutoresManmohan Singh, Maylene Briones, Gary Ott, Derek T. O’Hagan,
Tópico(s)T-cell and B-cell Immunology
ResumoAn approach involving the preparation of biodegradable microparticles with a cationic surface was developed to improve the delivery of adsorbed DNA into antigen-presenting cells after i.m. injection. The microparticles released intact and functional DNA over 2 weeks in vitro . In addition, the microparticles induced higher levels of marker gene expression in vivo . After i.m. immunization, the microparticles induced significantly enhanced serum antibody responses in comparison to naked DNA. Moreover, the level of antibodies induced by the microparticles was significantly enhanced by the addition of a vaccine adjuvant, aluminum phosphate. In addition, in contrast to naked DNA, the cationic microparticles induced potent cytotoxic T lymphocyte responses at a low dose.
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