Cofactor requirement of ribosome-inactivating proteins from plants
1997; Oxford University Press; Volume: 48; Issue: 8 Linguagem: Inglês
10.1093/jxb/48.8.1519
ISSN1460-2431
AutoresDomenica Carnicelli, Maurizio Brigotti, Paola Alvergna, Alessandra Pallanca, S Sperti, Lucio Montanaro,
Tópico(s)Transgenic Plants and Applications
ResumoA large number of type 1 ribosome-inactivating proteins (RIPs) from plants (families of Caryophyllaceae, Cucurbitaceae, Euphorbiaceae, Phytolaccaceae, and Poaceae) were examined for their requirement for ATP and supernatant factors for full activity. A marked requirement was observed with agrostin among Caryophyllaceae, gelonin among Euphorbiaceae, and with both barley RIP and tritin-S among Poaceae. The distribution of cofactor requirement in Phytolaccaceae discriminates leaf forms (cofactor-independent) from seed and root forms (cofactor-dependent). The results are discussed on the basis of the present knowledge on the tissue localization of RIPs and on the sensitivity of ribosomes to conspecific RIPs.
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