Paired related homeobox protein‐like 1 (Prrxl1) controls its own expression by a transcriptional autorepression mechanism
2014; Wiley; Volume: 588; Issue: 18 Linguagem: Inglês
10.1016/j.febslet.2014.08.006
ISSN1873-3468
AutoresCésar Bruno da Cunha Monteiro, Mariana Fontes da Costa, Carlos Reguenga, Deolinda Lima, Diogo S. Castro, Filipe Almeida Monteiro,
Tópico(s)Nerve injury and regeneration
ResumoThe homeodomain factor paired related homeobox protein‐like 1 (Prrxl1) is crucial for proper assembly of dorsal root ganglia (DRG)–dorsal spinal cord (SC) pain‐sensing circuit. By performing chromatin immunoprecipitation with either embryonic DRG or dorsal SC, we identified two evolutionarily conserved regions (i.e. proximal promoter and intron 4) of Prrxl1 locus that show tissue‐specific binding of Prrxl1. Transcriptional assays confirm the identified regions can mediate repression by Prrxl1, while gain‐of‐function studies in Prrxl1 expressing ND7/23 cells indicate Prrxl1 can down‐regulate its own expression. Altogether, our results suggest that Prrxl1 uses distinct regulatory regions to repress its own expression in DRG and dorsal SC.
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