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Validation of Intra-operative Detection of Paratracheal Lymph Node Metastasis Using Real-time RT-PCR Targeting Esophageal Squamous Cell Carcinoma

2003; Oxford University Press; Volume: 33; Issue: 11 Linguagem: Inglês

10.1093/jjco/hyg108

1465-3621

S. Nagatani,

Cancer-related gene regulation

Background: We previously reported that there was a significant correlation between paratracheal lymph node (LN) metastasis and cervical LN metastasis in thoracic esophageal squamous cell carcinoma (ESCC) patients. The purpose of this study was to establish an intra-operative detection method of LN micrometastasis (MM) of ESCC using hematoxylin–eosin (HE) staining, immunohistochemistry (IHC) and real-time RT–PCR with a Light Cycler technique, and to evaluate which method, or combination of methods, is most suitable for intra-operative detection of paratracheal LN MM.