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Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana

2003; American Association for the Advancement of Science; Volume: 301; Issue: 5633 Linguagem: Inglês

10.1126/science.1086391

1095-9203

José M. Alonso, Anna N. Stepanova, Thomas J. Leisse, Christopher J. Kim, Huaming Chen, Paul Shinn, Denise K. Stevenson, Justin Zimmerman, Pascual Barajas, Rosa Cheuk, Carmelita Gadrinab, Collen Heller, Albert Jeske, Eric Koesema, Cristina C. Meyers, Holly Parker, Lance Prednis, Yasser Ansari, Nathan Choy, Hashim Deen, M. Geralt, Nisha Hazari, Emily Hom, Meagan Karnes, Celene B. Mulholland, Ral Ndubaku, Ian Schmidt, Plinio Guzmán, Laura Aguilar‐Henonin, Markus Schmid, Detlef Weigel, David E. Carter, Trudy Marchand, Eddy Risseeuw, Debra Brogden, Albana Zeko, William L. Crosby, Charles C. Berry, Joseph R. Ecker,

CRISPR and Genetic Engineering

Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the ∼29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.