CD69 expression as an index of T-cell function: assay standardization, validation and use in monitoring immune recovery
2007; Elsevier BV; Volume: 9; Issue: 2 Linguagem: Inglês
10.1080/14653240601182838
ISSN1477-2566
AutoresW.B. Lindsey, Mark W. Lowdell, Gerald E. Marti, Fatima Abbasi, Vincent E. Zenger, Kevin King, Lawrence S. Lamb,
Tópico(s)HIV Research and Treatment
ResumoBackground CD69 is a surrogate marker of T-cell responsiveness to mitogen and Ag stimulus and can be used as a measure of T-lymphocyte activation. Quantitative flow cytometric determination of CD69 expression on T lymphocytes has several advantages over traditional lymphocyte proliferation assays, but this method has not yet been standardized for clinical applications. Methods We qualified a commercially available assay using the manufacturer's procedures for measurement of T-cell response to a mitogen (PHA), superantigen ( Staphylococcus endotoxin B; SEB) and Ca 2+ ionophore (phorbyl myristate acetate; PMA) with peripheral blood from healthy volunteers. Following this, we tested the usefulness of the assay in determining T-cell responses to PHA and SEB for six immunocompromised patients. Results Healthy volunteers showed 17-fold increases in T-cell CD69 Ab bound per cell (ABC) with PHA stimulation compared with the baseline. SEB was also an effective T-cell activating agent, increasing CD69 ABC by 5-fold, comparable with results obtained with PMA stimulation. PHA- and SEB-stimulated T-cell CD69 ABC for patients 100 days post-BM transplant were generally below 1 SD of that from healthy volunteers. SEB-stimulated T-cell CD69 expression was significantly depressed for CD8 + T cells while CD4 + T-cell responses to SEB were generally within 1 SD of the mean for healthy volunteers. Discussion These results suggest that quantitative measurement of CD69 surface expression by flow cytometry is a useful diagnostic tool for detailed assessment of T-lymphocyte and subset activation.
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