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Establishment of an agamid cell line and isolation of adenoviruses from central bearded dragons ( Pogona vitticeps )

2014; SAGE Publishing; Volume: 26; Issue: 2 Linguagem: Inglês

10.1177/1040638714523615

1943-4936

Inna Ball, Marc Hoferer, Rachel E. Marschang,

Turtle Biology and Conservation

A cell line was established from whole 6–8-week-old central bearded dragon ( Pogona vitticeps) embryos. Cells were mid-sized and showed an elongated and polymorphic form. The cell line grew in a monolayer and has been serially passaged for 17 passages at time of publication. This cell line has been used with samples from adenovirus polymerase chain reaction (PCR)-positive bearded dragons, and 2 virus isolates have been obtained so far. The isolates show a clear cytopathic effect in inoculated cells. Both virus isolates have been serially passaged on this cell line, and have been identified by PCR amplification and sequencing of a portion of the DNA-dependent DNA polymerase gene and show 100% nucleotide identity to the corresponding region of an agamid adenovirus. Electron microscopic examination of supernatant from infected cells demonstrated the presence of nonenveloped particles, with a diameter of approximately 80 nm in both virus isolates.