Induction of puberty by autograft of cryopreserved ovarian tissue
2012; Elsevier BV; Volume: 379; Issue: 9815 Linguagem: Inglês
10.1016/s0140-6736(11)61781-9
ISSN1474-547X
AutoresCatherine Poirot, Fadi Abirached, Marie Prades, Christiane Coussieu, Françoise Bernaudin, P. Piver,
Tópico(s)Pluripotent Stem Cells Research
ResumoIn November, 2003, a 10-year-old girl with severe homozygous sickle-cell disease was referred to our reproductive biology unit requesting cryopreservation of ovarian tissue before a myeloablative conditioning regimen followed by allogeneic haemopoietic stem-cell transplantation. 1 Bernaudin F Socie G Kuentz M et al. Long-term results of related myeloablative stem-cell transplantation to cure sickle cell disease. Blood. 2007; 110: 2749-2756 Crossref PubMed Scopus (379) Google Scholar Ovarian failure is a common adverse effect after such conditioning. 2 Thibaud E Rodriguez-Macias K Trivin C Esperou H Michon J Brauner R Ovarian function after bone marrow transplantation during childhood. Bone Marrow Transplant. 1998; 21: 287-290 Crossref PubMed Scopus (143) Google Scholar She was 142 cm tall, weighed 31 kg, and had no signs of puberty. Right oophorectomy was done by laparoscopy under general anaesthesia. The ovarian tissue was transferred to the reproductive laboratory where the cortex was isolated and dissected into 23 ovarian fragments, which were cryopreserved by the slow cooling protocol. 3 Gosden R Baird DT Wade JC Webb R Restoration of fertility to oophorectomized sheep by ovarian autografts stored at −196 degrees C. Hum Reprod. 1994; 9: 597-603 PubMed Google Scholar Microscopic analysis of one ovarian fragment showed a density of 8·6 primordial follicles per mm2.
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