Synthesis of Rat Liver Albumin in a Rabbit Reticulocyte Cell-free Protein-synthesizing System
1973; Elsevier BV; Volume: 248; Issue: 22 Linguagem: Inglês
10.1016/s0021-9258(19)43241-9
ISSN1083-351X
AutoresJohn M. Taylor, Robert Schimke,
Tópico(s)RNA Research and Splicing
ResumoAbstract Rat liver polysomal RNA can direct the synthesis of albumin in a cell-free system derived from rabbit reticulocytes. The in vitro product, labeled with [3H]leucine, was identified as albumin by precipitation with specific antibody and by comparison to authentic serum [14C]albumin. The in vitro product and serum albumin co-migrate during electrophoresis in sodium dodecyl sulfate polyacrylamide gels; they have identical immunoprecipitation curves; and their peptides show a precise correspondence in the ion exchange chromatography of tryptic digests. Albumin messenger RNA has a sedimentation coefficient of approximately 18 S in sucrose gradients and it appears to contain a polyadenylate segment. Polysomal RNA can be enriched for albumin synthesizing activity by selective adsorption to nitrocellulose filters.
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