Artigo Acesso aberto Revisado por pares

Re-evaluation of total sulphur amino acid requirement and determination of replacement value of cystine for methionine in semi-purified diets of juvenile Nile tilapia, Oreochromis niloticus

2008; Wiley; Volume: 15; Issue: 3 Linguagem: Inglês

10.1111/j.1365-2095.2008.00589.x

ISSN

1365-2095

Autores

T. N. A. Nguyen, D. Allen Davis,

Tópico(s)

Child Nutrition and Water Access

Resumo

Aquaculture NutritionVolume 15, Issue 3 p. 247-253 Re-evaluation of total sulphur amino acid requirement and determination of replacement value of cystine for methionine in semi-purified diets of juvenile Nile tilapia, Oreochromis niloticus T.N. NGUYEN, T.N. NGUYEN Faculty of Fisheries, Nong Lam University, Linh Trung Ward, Thu Duc district, Ho Chi Minh City, Viet NamSearch for more papers by this authorD.A. DAVIS, D.A. DAVIS Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL, USASearch for more papers by this author T.N. NGUYEN, T.N. NGUYEN Faculty of Fisheries, Nong Lam University, Linh Trung Ward, Thu Duc district, Ho Chi Minh City, Viet NamSearch for more papers by this authorD.A. DAVIS, D.A. DAVIS Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL, USASearch for more papers by this author First published: 07 May 2009 https://doi.org/10.1111/j.1365-2095.2008.00589.xCitations: 29 Tri N. Nguyen, Faculty of Fisheries, Nong Lam University, Linh Trung ward, Thu Duc district, Ho Chi Minh city, Viet Nam. E-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Two feeding experiments were conducted to re-evaluate the total sulphur amino acid (TSAA; methionine and cystine) requirement and determine the replacement value of cystine for methionine of juvenile Nile tilapia (Oreochromis niloticus). Semi-purified diets used in both experiments contained 3510 kcal gross energy and 280 g of protein per kilogram diet from casein, gelatin and crystalline amino acids. The basal diet of the first experiment contained 3.1 g methionine and 0.4 g cystine per kilogram. l-methionine was added to the seven remaining diets at 1.0 g kg−1 increment to produce methionine levels ranging from 3.1 to 10.1 g kg−1 diet. Each diet was fed to four replicate groups of juvenile Nile tilapia (1.28 g mean weight) in a recirculation system for 8 weeks. Broken-line regression analysis of weight gain data indicated that the TSAA requirement of juvenile Nile tilapia was 8.5 g kg−1 of the diet or 30.4 g kg−1 of dietary protein. In the second experiment, TSAA level was set at 95% of the requirement value determined in the first experiment. Seven diets were made with different ratios of l-methionine and l-cystine (20 : 80, 30 : 70, 40 : 60, 50 : 50, 60 : 40, 70 : 30 and 80 : 20, based on an equimolar sulphur basis). Each diet was also fed to four replicate groups of juvenile Nile tilapia (4.14 g mean weight) in a recirculation system for 8 weeks. Regression analysis of weight gain data using broken-line model indicated that cystine (on a molar sulphur basis) could replace up to 49% of methionine requirement in semi-purified diets for juvenile Nile tilapia. References Abdelghany, A.E. (2000) Optimum dietary protein requirements for Oreochromis niloticus L. fry using formulated semi-purified diets. in: Tilapia Culture in the 21st Century (Fitzsimmons, K. & Filho, J.C. eds), pp. 101– 108. Proceedings of the Fifth International Symposium on Tilapia in Aquaculture, Rio de Janeiro, Brazil; American Tilapia Association, Charles Town, West Virginia; and ICLARM, Penang, Malaysia. 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