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Leptin and Its Receptor in Normal Human Gastric Mucosa and in Helicobacter pylori -Associated Gastritis

1999; Taylor & Francis; Volume: 34; Issue: 10 Linguagem: Inglês

10.1080/003655299750025039

1502-7708

Matthias Breidert, Stefan Miehlke, Annegret Glasow, Zsolt Orban, M. Stolte, G. Ehninger, E. Bayerdörffer, O Nettesheim, U. Halm, A. Haidan, Stefan R. Bornstein,

Helicobacter pylori-related gastroenterology studies

Background: Leptin, a newly discovered weight-reducing hormone, is mainly produced in fat cells. Recently, this hormone has been reported to be produced in rat gastric mucosa cells. In the present study we analyzed the localization and expression of leptin and its receptors in normal human gastric mucosa and in patients with Helicobacter pylori-associated gastritis. Methods: Plasma leptin levels and gastric mucosa leptin content were determined in 39 patients with dyspepsia. Cellular localization of leptin and of the signaling receptor (Ob-RL) were assessed by immunohistochemistry. Reverse transcriptase polymerase chain reaction (RT-PCR) for leptin receptor isoforms was performed on gastric epithelial cells isolated by laser-capture-microdissection. Results: Leptin content of the corpus gastric mucosa in H. pylori