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Positional Cloning of a Gene for Nematode Resistance in Sugar Beet

1997; American Association for the Advancement of Science; Volume: 275; Issue: 5301 Linguagem: Inglês

10.1126/science.275.5301.832

1095-9203

Daguang Cai, Michael Kleine, Sirak Kifle, Hans‐Joachim Harloff, Niels Sandal, Kjeld A. Marcker, R. M. Klein-Lankhorst, Elma M. J. Salentijn, W. Lange, Willem J. Stiekema, Urs Wyss, Florian M. W. Grundler, Christian Jung,

Plant Virus Research Studies

The Hs1 pro-1 locus confers resistance to the beet cyst nematode ( Heterodera schachtii Schmidt), a major pest in the cultivation of sugar beet ( Beta vulgaris L.). The Hs1 pro-1 gene was cloned with the use of genome-specific satellite markers and chromosomal break-point analysis. Expression of the corresponding complementary DNA in a susceptible sugar beet conferred resistance to infection with the beet cyst nematode. The native Hs1 pro-1 gene, expressed in roots, encodes a 282-amino acid protein with imperfect leucine-rich repeats and a putative membrane-spanning segment, features similar to those of disease resistance genes previously cloned from higher plants.