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CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans

2013; Oxford University Press; Volume: 195; Issue: 3 Linguagem: Inglês

10.1534/genetics.113.156299

1943-2631

Selma Waaijers, Vincent Portegijs, Jana Kerver, Bennie Lemmens, Marcel Tijsterman, Sander van den Heuvel, Mike Boxem,

Pluripotent Stem Cells Research

Abstract The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.