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Detection of Antibodies to Babesia equi in Horses by a Latex Agglutination Test Using Recombinant EMA-1

2001; American Society for Microbiology; Volume: 8; Issue: 3 Linguagem: Inglês

10.1128/cdli.8.3.645-646.2001

1098-6588

Xuenan Xuan, Ikuo Igarashi, Tetsuya Tanaka, Shinya Fukumoto, Hideyuki Nagasawa, Kozo Fujisaki, Takeshi Mikami,

Toxin Mechanisms and Immunotoxins

ABSTRACT A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly between sera from B. equi -infected horses and sera from Babesia caballi -infected horses or from normal horses. The LAT results were identical to those of a previously developed enzyme-linked immunosorbent assay. These results indicate that LAT using recombinant EMA-1 might be very useful as a routine screening method for the diagnosis of B. equi infection.