Portal de Periódicos da CAPES

Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them

2011; Wiley; Volume: 26; Issue: 1 Linguagem: Inglês

10.1096/fj.11-181941

1530-6860

Jésica Díaz‐Vera, Marcial Camacho, José David Machado, Natalia Domínguez, Mónica S. Montesinos, Juan R. Hernández‐Fernaud, Rafael Luján, Ricardo Borges,

Biotin and Related Studies

The FASEB JournalVolume 26, Issue 1 p. 430-438 Research CommunicationFree to Read Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them Jésica Díaz-Vera, Jésica Díaz-Vera Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorMarcial Camacho, Marcial Camacho Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorJosé David Machado, José David Machado Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorNatalia Domínguez, Natalia Domínguez Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorMónica S. Montesinos, Mónica S. Montesinos Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorJuan R. Hernández-Fernaud, Juan R. Hernández-Fernaud Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorRafael Luján, Rafael Luján Departamento de Ciencias Médicas, Facultad de Medicina, Universidad de Castilla-La Mancha, Campus Biosanitario, Albacete, SpainSearch for more papers by this authorRicardo Borges, Corresponding Author Ricardo Borges rborges@ull.es Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainCorrespondence: Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, E-38071 La Laguna, Tenerife, Spain. E-mail: rborges@ull.esSearch for more papers by this author Jésica Díaz-Vera, Jésica Díaz-Vera Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorMarcial Camacho, Marcial Camacho Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorJosé David Machado, José David Machado Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorNatalia Domínguez, Natalia Domínguez Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorMónica S. Montesinos, Mónica S. Montesinos Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorJuan R. Hernández-Fernaud, Juan R. Hernández-Fernaud Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainSearch for more papers by this authorRafael Luján, Rafael Luján Departamento de Ciencias Médicas, Facultad de Medicina, Universidad de Castilla-La Mancha, Campus Biosanitario, Albacete, SpainSearch for more papers by this authorRicardo Borges, Corresponding Author Ricardo Borges rborges@ull.es Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, SpainCorrespondence: Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, E-38071 La Laguna, Tenerife, Spain. E-mail: rborges@ull.esSearch for more papers by this author First published: 11 October 2011 https://doi.org/10.1096/fj.11-181941Citations: 9 This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat ABSTRACT Chromogranins are the main soluble proteins in the large dense core secretory vesicles (LDCVs) found in aminergic neurons and chromaffin cells. We recently demonstrated that chromogranins A and B each regulate the concentration of adrenaline in chromaffin granules and its exocytosis. Here we have further studied the role played by these proteins by generating mice lacking both chromogranins. Surprisingly, these animals are both viable and fertile. Although chromogranins are thought to be essential for their biogenesis, LDCVs were evident in these mice. These vesicles do have a somewhat atypical appearance and larger size. Despite their increased size, single-cell amperometry recordings from chromaffin cells showed that the amine content in these vesicles is reduced by half. These data demonstrate that although chromogranins regulate the amine concentration in LDCVs, they are not completely essential, and other proteins unrelated to neurosecretion, such as fibrinogen, might compensate for their loss to ensure that vesicles are generated and the secretory pathway conserved.—Díaz-Vera, J., Camacho, M., Machado, J. D., Domínguez, N., Montesinos, M. S., Hernández-Fernaud, J. R., Luján, R., Borges, R. Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them. FASEB J. 26, 430–438 (2012). www.fasebj.org Citing Literature Supporting Information Filename Description fsb2fj11181941-sup-0001-S1.doc5.8 MB Supporting Information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume26, Issue1January 2012Pages 430-438 RelatedInformation