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First Report of Myrothecium roridum Causing Myrothecium Leaf Spot on Begonia in Brazil
2015; American Phytopathological Society; Volume: 100; Issue: 3 Linguagem: Inglês
10.1094/pdis-09-15-1097-pdn
ISSN1943-7692
AutoresMiriam Fumiko Fujinawa, Nadson de Carvalho Pontes, Helson Mário Martins do Vale, Natércia Santos, B. de A. Halfeld-Vieira,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 100, No. 3First Report of Myrothecium roridum Causing Myrothecium Leaf Spot on Begonia in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Myrothecium roridum Causing Myrothecium Leaf Spot on Begonia in BrazilM. F. Fujinawa, N. C. Pontes, H. M. M. do Vale, N. F. Santos, and B. A. Halfeld-VieiraM. F. FujinawaSearch for more papers by this author, N. C. PontesSearch for more papers by this author, H. M. M. do ValeSearch for more papers by this author, N. F. SantosSearch for more papers by this author, and B. A. Halfeld-Vieirahttp://orcid.org/0000-0003-4115-0155Search for more papers by this authorAffiliationsAuthors and Affiliations M. F. Fujinawa N. C. Pontes , Agronomia, Instituto Federal Goiano, Morrinhos, Goiás 75650-000, Brazil H. M. M. do Vale , Departamento de Fitopatologia, Universidade de Brasília, Brasília 70910-900, Brazil N. F. Santos B. A. Halfeld-Vieira , Embrapa Meio Ambiente, Jaguariúna, São Paulo 13820-000, Brazil. Published Online:29 Jan 2016https://doi.org/10.1094/PDIS-09-15-1097-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Begonia (Begonia elatior) cultivars are among the most commercialized ornamental potted plants in Brazil. Since 2012, a high incidence of plants with leaf spots has been found in greenhouses of São Paulo State, for the first time in Jacareí Municipality (23°15′58.45″ S; 46°2′32.24″ W). The symptoms, initially small and circular, evolve into irregularly shaped spots with a light brown color. Dark sporodochia surrounded by white hyphal tufts develop in older lesions. Symptoms were consistent with those of Myrothecium leaf spot observed on other ornamental plants such as gardenia, gloxinias, impatiens, and begonias in North America (Daughtrey et al. 1995). Isolations from these lesions on potato dextrose agar (PDA) produced white, floccose, concentric-ringed colonies with irregular shapes of dark green-to-black sporodochia. Conidiophores presented 2 to 4 branches at each node while phialides were hyaline, cylindrical, in whorls of 3 to 5, and measured 13 to 16 × 2.0 μm. Conidia were hyaline, one-celled, rod-shaped with rounded ends, and measured 5 to 7.5 × 2.0 μm. These characteristics are consistent with the morphology of Myrothecium roridum Tode (Tulloch 1972). Sequencing of the internal transcribed spacer regions ITS1, 5.8S rDNA, and ITS2 of one Myrothecium isolate was performed using ITS5 (5′-GGAAGTAAAAGTCGTAACAAGG-3′) and ITS4 (5′- TCCTCCGCTTATTGATATGC-3′) primers (White et al. 1990) and compared with sequences in the GenBank database. The resulting 563-bp amplicon (Accession No. KJ494661) was 99% identical to the other M. roridum sequences present on GenBank (JF343832 and KJ174523.1). The strain was deposited in the Mycological Reference Collection of the University of Brasília (Accession No. KJ4946612246). To confirm pathogenicity, 10 begonia plants were inoculated, placing mycelial plugs on begonia leaves, from which five were previously injured with a needle tip. Plants were maintained for 7 days at 28°C at relative humidity above 80%. Ten control plants received only PDA disks. Symptoms were observed on all inoculated plants after 7 days, while control plants remained symptomless. M. roridum was consistently reisolated from symptomatic tissues, fulfilling Koch’s postulates. This is the first report of Myrothecium leaf spot caused by M. roridum on begonias in Brazil. This pathogen has been previously reported on begonias in North America (Daughtrey et al. 1995). Begonia represents a popular ornamental plant in Brazil and damage to the foliage reduces its marketability; therefore, early and correct diagnosis is necessary to take control measures and reduce production losses.References:Daughtrey, M. G., et al. 1995. Page 19 in: Compendium of Flowering Potted Plant Diseases. APS Press, St. Paul, MN. Google ScholarTulloch, M. 1972. Mycol. Pap. 130:1. Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis, et al., eds. Academic Press, San Diego, CA. Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 3 March 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 2 Mar 2016Published: 29 Jan 2016First Look: 2 Nov 2015Accepted: 27 Oct 2015 Pages: 655-655 Information© 2016 The American Phytopathological SocietyCited byMyrothecium roridum (blight: eggplant)CABI Compendium, Vol. CABI CompendiumXepicula yifeii sp. nov. caused a leaf blight of Lasia spinosa (Araceae) in South China karst27 June 2020 | European Journal of Plant Pathology, Vol. 158, No. 1Biological control of gray mold and Myrothecium leaf spot in begoniasCrop Protection, Vol. 133Characterization of the fungal community in the canopy air of the invasive plant Ageratina adenophora and its potential to cause plant diseases26 March 2020 | PLOS ONE, Vol. 15, No. 3Emergence of Leaf Spot Disease on Leafy Vegetable and Ornamental Crops Caused by Paramyrothecium and Albifimbria SpeciesSlavica Matić, Giovanna Gilardi, Maria Lodovica Gullino, and Angelo Garibaldi24 April 2019 | Phytopathology®, Vol. 109, No. 6First Report of a Leaf Spot Caused by Paramyrothecium roridum on Tectona grandis in BrazilR. 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