Regulated expression of human Aγ-, β-, and hybrid γβ-globin genes in transgenic mice: Manipulation of the developmental expression patterns
1986; Cell Press; Volume: 46; Issue: 1 Linguagem: Inglês
10.1016/0092-8674(86)90862-7
ISSN1097-4172
AutoresGeorge Kollias, Nick Wrighton, J. Hurst, Frank Grosveld,
Tópico(s)RNA and protein synthesis mechanisms
ResumoWe have introduced the human fetal γ- and adult β-globin genes into the germ line of mice. Analysis of the resulting transgenic mice shows that the human γ-globin gene is expressed like an embryonic mouse globin gene; the human β-globin gene is expressed (as previously shown) like an adult mouse globin gene. These results imply that the regulatory signals for tissue- and developmental stage-specific expression of the globin genes have been conserved between man and mouse but that the timing of the signals has changed. Because the two genes are expressed differently, we introduced a hybrid γβ-globin gene construct. The combination of the regulatory sequences resulted in the expression of the hybrid gene at all stages in all the murine erythroid tissues.
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