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Asian sand dust causes subacute peripheral immune modification with NF-κB activation

2013; Wiley; Volume: 30; Issue: 5 Linguagem: Inglês

10.1002/tox.21931

1522-7278

Yuan Song, Takamichi Ichinose, Kentaro Morita, Tsukasa Nakanishi, Tamotsu Kanazawa, Yasuhiro Yoshida,

Biomarkers in Disease Mechanisms

Environmental ToxicologyVolume 30, Issue 5 p. 549-558 Research Article Asian sand dust causes subacute peripheral immune modification with NF-κB activation Yuan Song, Yuan Song Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanSearch for more papers by this authorTakamichi Ichinose, Takamichi Ichinose Department of Health Sciences, Oita University of Nursing and Health Sciences, 2944-9 Notsuharu, Oita, 870-1201 JapanSearch for more papers by this authorKentaro Morita, Kentaro Morita Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanSearch for more papers by this authorTsukasa Nakanishi, Tsukasa Nakanishi Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanSearch for more papers by this authorTamotsu Kanazawa, Tamotsu Kanazawa Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanSearch for more papers by this authorYasuhiro Yoshida, Corresponding Author Yasuhiro Yoshida Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanCorrespondence to: Y. Yoshida; e-mail: freude@med.uoeh-u.ac.jpSearch for more papers by this author Yuan Song, Yuan Song Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanSearch for more papers by this authorTakamichi Ichinose, Takamichi Ichinose Department of Health Sciences, Oita University of Nursing and Health Sciences, 2944-9 Notsuharu, Oita, 870-1201 JapanSearch for more papers by this authorKentaro Morita, Kentaro Morita Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanSearch for more papers by this authorTsukasa Nakanishi, Tsukasa Nakanishi Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanSearch for more papers by this authorTamotsu Kanazawa, Tamotsu Kanazawa Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanSearch for more papers by this authorYasuhiro Yoshida, Corresponding Author Yasuhiro Yoshida Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555 JapanCorrespondence to: Y. Yoshida; e-mail: freude@med.uoeh-u.ac.jpSearch for more papers by this author First published: 05 December 2013 https://doi.org/10.1002/tox.21931Citations: 10 The authors declare they have no competing financial interests. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat ABSTRACT Asian sand dust (ASD), a type of particulate matter found in Asia, migrates to East Asia. The increased airborne spread of ASD has led to concerns regarding possible adverse health effects. Our group previously reported that ASD induces lung inflammation in mice, but it is still unclear whether ASD affects lymphoid organs. In this study, we investigated the effect of ASD on splenocytes in a mouse model of ASD exposure. ICR mice were intratracheally administered a single dose of normal saline (control) or ASD and were subsequently sacrificed 1 or 3 days later. TNF-α production in bronchoalveolar lavage fluids was higher at day 1, but not at day 3, after ASD administration. The enzyme-linked immunosorbent assay results showed that ASD administration increased mitogen-induced IL-2, TNF-α, and IL-6 production in splenocytes. Additionally, cell viability assay showed enhanced splenocyte proliferation at day 3, but not at day 1, after ASD administration. The electrophoretic mobility shift assay results demonstrated that nuclear factor κB (NF-κB) was activated in splenocytes on day 3, but not on day 1. In particular, NF-κB activation was detected in CD4+ and CD11b+ cells on day 3. These results suggest that ASD induces subacute inflammatory responses with NF-κB activation in the spleen, in contrast to acute inflammation in the lungs. © 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 549–558, 2015. Citing Literature Volume30, Issue5May 2015Pages 549-558 RelatedInformation