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Chemical Structure of Antithrombin-active Rhamnan Sulfate from Monostrom nitidum

1998; Oxford University Press; Volume: 62; Issue: 9 Linguagem: Inglês

10.1271/bbb.62.1647

1347-6947

Naoki Harada, Masaakira Maeda,

Proteoglycans and glycosaminoglycans research

Rhamnan sulfate was extracted with boiling water from the cell wall of Monostroma nitridum, and the resulting extract was purified by ion-exchange and size-exclusion chromatography. The polysaccharide, which is regarded as a homopolysaccharide, was 6-fold more antithrombin-active than the heparin standard. The antithrombin activity was decreased, by desulfation, although the non-active product still retained 8% of the sulfate ester. A comparative structural study of the intact rhamnan sulfate and rhamnan, its desulfated product, by periodate oxidation, Smith degradation and methylation analysis revealed the rhamnan sulfate to consist of alpha-1,3-linked L-rhamnose residues, some of which were substituted with sulfate groups mainly at position O-2. Minor amounts of internal 1,2-linked rhamnose and branched rhamnose linkages were also detected.