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Solid-Phase Peptide Synthesis

1998; Humana Press; Linguagem: Inglês

10.1007/978-1-59259-642-3_42

1949-2456

Gregg Fields,

Machine Learning in Bioinformatics

Methods for synthesizing peptides are divided conveniently into two categories solution and solid-phase (SPPS) Solution synthesis retains value in large-scale manufacturing and for specialized laboratory applications. However, the need to optimize reaction conditions, yields, and purification procedures for essentially every intermediate (each of which has unpredictable solubility and crystallization characteristics) renders solution methods time-consuming and labor-intensive Consequently, most workers now requiring peptides for then research opt for the more accessible solidphase approach In this chapter, an overview of SPPS is presented. For brevity, only commercially available reagents and derivatives utilized for synthesis will be considered here The reader 1s referred to a number of excellent, comprehensive reviews (1–9) for further discussion of the solid-phase technique.