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Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

2014; Research Foundation of Ribeirão Preto; Volume: 13; Issue: 4 Linguagem: Inglês

10.4238/2014.november.11.5

1676-5680

Marília Lazarotto, Paola Mendes Milanesí, Marlove Fátima Brião Muniz, Lia Rejane Silveira Reiniger, Rafael Beltrame, Ricardo Harakava, Elena Blume,

Mycorrhizal Fungi and Plant Interactions

Fusarium spp pathogenic to pecan tree seeds of symptomatic plants (leaf necrosis or root rot) were used for pathogenicity tests.Morphological characterization was conducted using only pathogenic isolates, for a total of 11 isolates, based on the mycelial growth rate, sporulation, colony pigmentation, and conidial length and width variables.Pathogenic isolates were grouped based on morphological characteristics, and molecular characterization was performed by sequencing TEF-1α genes.Pathogenic isolates belonging to the Fusarium chlamydosporum species complex, Fusarium graminearum species complex, Fusarium proliferatum, and Fusarium oxysporum were identified based on the TEF-1α region.Morphological characteristics were used to effectively differentiate isolates and group the isolates according to genetic similarity, particularly conidial width, which emerged as a key morphological descriptor in this study.