Portal de Periódicos da CAPES

Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators

2008; Elsevier BV; Volume: 69; Issue: 6 Linguagem: Inglês

10.1016/j.phytochem.2008.01.017

1873-3700

Lie‐Fen Shyur, Chi‐Chang Huang, Chiu‐Ping Lo, Chih‐Yang Chiu, Yi‐Ping Chen, Sheng‐Yang Wang, Shang‐Tzen Chang,

Toxin Mechanisms and Immunotoxins

Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl4-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC50 = 5 μg/mL) and TPA-induced COX-2 protein expression in mouse skin (1 mg/200 μL/site). CJH7-2 (10 mg/kg BW) can prevent CCl4-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection.