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The application of single cell gel electrophoresis or comet assay to human monitoring studies

1999; Instituto Nacional de Salud Pública; Volume: 41; Linguagem: Inglês

10.1590/s0036-36341999000800007

1606-7916

Mahara Valverde, Patricia Ostrosky‐Wegman, Emilio Rojas, Teresa I. Fortoul van der Goes, Fernando Meneses, Matiana Ramírez, Fernando Díaz‐Barriga, Mariano E. Cebrián,

Health, Environment, Cognitive Aging

Objective. In the search of new human genotoxic biomarkers, the single cell gel electrophoresis assay has been proposed as a sensible alternative. Material and methods. This technique detects principally single strand breaks as well as alkali-labile and repair-retarded sites. Results. Herein we present our experience using the single cell gel electrophoresis assay in human population studies, both occupationally and environmentally exposed. Conclusions. We discuss the assay feasibility as a genotoxic biomarker.