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Artigo Revisado por pares
BIBTEX RIS

A Reducing and Oxidizing System Minimizes Photobleaching and Blinking of Fluorescent Dyes

2008; Wiley; Volume: 47; Issue: 29 Linguagem: Inglês

10.1002/anie.200801518

ISSN

1521-3773

Autores

Jan Vogelsang, Robert Kasper, Christian Steinhauer, Britta Person, Mike Heilemann, Markus Sauer, Philip Tinnefeld,

Tópico(s)

Advanced biosensing and bioanalysis techniques

Resumo

On the ROXS: Photobleaching represents one of the main limitations of modern fluorescence microscopy. A reducing and oxidizing system (ROXS) has been introduced that recovers triplet states as well as charge-separated states through electron-transfer reactions (see picture). Thus the blinking and photobleaching of fluorophores is strikingly reduced.

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