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Improved Specificity of a Multiplex Immunoassay for Quantitation of Anti-Diphtheria Toxin Antibodies with the Use of Diphtheria Toxoid

2011; American Society for Microbiology; Volume: 18; Issue: 7 Linguagem: Inglês

10.1128/cvi.05081-11

1556-6811

Pieter G. M. van Gageldonk, Christina von Hunolstein, Fiona van der Klis, Guy A. M. Berbers,

Clostridium difficile and Clostridium perfringens research

ABSTRACT A nonspecific binding of antibodies to diphtheria toxin, especially in adult serum samples, was observed in our diphtheria-tetanus-pertussis multiplex immunoassay (DTaP4 MIA). This can be significantly reduced by the use of diphtheria toxoid, achieving a good correlation with the Vero cell neutralization test and the toxin binding inhibition assay.