Novel Strong Cation‐exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC
2008; Wiley; Volume: 26; Issue: 11 Linguagem: Inglês
10.1002/cjoc.200890368
ISSN1614-7065
AutoresGeng‐Liang YANG, Ligai Bai, Hai‐Yan LIU, Yu Zhao, Fengxin Li, Weimin Cao,
Tópico(s)Innovative Microfluidic and Catalytic Techniques Innovation
ResumoChinese Journal of ChemistryVolume 26, Issue 11 p. 2063-2066 Full Paper Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC Geng-Liang YANG, Geng-Liang YANG ygl@hbu.edu.cn College of Pharmacy, Hebei University, Baoding, Hebei 071002, China Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China Tel.: 0086-0312-5079788; Fax: 0086-0312-5971107Search for more papers by this authorLi-Gai BAI, Li-Gai BAI College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this authorHai-Yan LIU, Hai-Yan LIU College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this authorYu ZHAO, Yu ZHAO College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this authorFeng-Xin LI, Feng-Xin LI College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this authorWei-Min CAO, Wei-Min CAO College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this author Geng-Liang YANG, Geng-Liang YANG ygl@hbu.edu.cn College of Pharmacy, Hebei University, Baoding, Hebei 071002, China Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China Tel.: 0086-0312-5079788; Fax: 0086-0312-5971107Search for more papers by this authorLi-Gai BAI, Li-Gai BAI College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this authorHai-Yan LIU, Hai-Yan LIU College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this authorYu ZHAO, Yu ZHAO College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this authorFeng-Xin LI, Feng-Xin LI College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this authorWei-Min CAO, Wei-Min CAO College of Pharmacy, Hebei University, Baoding, Hebei 071002, ChinaSearch for more papers by this author First published: 25 November 2008 https://doi.org/10.1002/cjoc.200890368Citations: 7 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract A novel cation-exchange polymeric monolith based on poly(vinyl ester resin) monolith was applied to separation in our present work. Poly- (vinyl ester resin) was as the monomer as well as the crosslinking agent in the polymerization which was induced by the initiator AIBN (2-methylpropropionitrile). 1-Hexadecanol was used as the porogen and methanol was used to make the 1-hexadecanol resolve easier. The short (1 cm) monolithic column was used to separate Lys from chicken egg white, and high resolution was obtained in this experiment. The results suggested that such kind of monolithic column could be used as a simple, cheap, effective method for the separation and purification of Lys. It is a good kind of stationary phase for HPLC. Citing Literature Volume26, Issue11November, 2008Pages 2063-2066 RelatedInformation
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