Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests
2001; Microbiology Society; Volume: 50; Issue: 8 Linguagem: Inglês
10.1099/0022-1317-50-8-663
ISSN1473-5644
AutoresT. Marrodan, R. NENOVA-POLIAKOVA, M. Rubio, Javier Ariza, Encarnación Clavijo, H. L. Smits, Ramón Díaz,
Tópico(s)Galectins and Cancer Biology
ResumoThe results of a dipstick assay for the detection of immunoglobulin M (IgM) to Brucella smooth lipopolysaccharide (S-LPS) correlated with those of an enzyme-linked immunosorbent assay (ELISA) for IgM and of the serum agglutination test (SAT) performed with and without dithiothreitol. Two sera which were dithiothreitol-sensitive and were dipstick negative were shown to contain specific IgA. The dipstick assay is recommended as a simple method for detecting specific IgM antibodies in acute-phase brucellosis patients.
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