The 39 Steps: A Cautionary Tale of Quantitative 3-D Fluorescence Microscopy
2000; Future Science Ltd; Volume: 28; Issue: 5 Linguagem: Inglês
10.2144/00285bt01
ISSN1940-9818
Autores Tópico(s)Single-cell and spatial transcriptomics
ResumoBioTechniquesVol. 28, No. 5 BioImagingOpen AccessThe 39 Steps: A Cautionary Tale of Quantitative 3-D Fluorescence MicroscopyJim PawleyJim Pawley*Address correspondence to Dr. Jim Pawley, Department of Zoology, University of Wisconsin-Madison, 1525 Linden Drive, Madison, WI 53705, USA. Internet: E-mail Address: jbpawley@facstaff.wisc.eduUniversity of Wisconsin-Madison, Madison, WI, USAPublished Online:29 Aug 2018https://doi.org/10.2144/00285bt01AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit FiguresReferencesRelatedDetailsCited By3D confocal imaging methodology optimized for pore space characterization of carbonatesEngineering Geology, Vol. 13Laminar flow-based microfluidic systems for molecular interaction analysis—Part 1: Chip development, system operation and measurement setupLaminar flow-based microfluidic systems for molecular interaction analysis—Part 2: Data extraction, processing and analysisMatrix glycoconjugate characterization in multispecies biofilms and bioaggregates from the environment by means of fluorescently-labeled lectins8 August 2022 | Frontiers in Microbiology, Vol. 13Quantitative Stimulated Raman Scattering Microscopy: Promises and PitfallsAnnual Review of Analytical Chemistry, Vol. 15, No. 1Using FDA-approved drugs as off-label fluorescent dyes for optical biopsies: from in silico design to ex vivo proof-of-concept4 June 2021 | Methods and Applications in Fluorescence, Vol. 9, No. 3Cryptochrome 1a localisation in light- and dark-adapted retinae of several migratory and non-migratory bird species: no signs of light-dependent activation21 February 2021 | Ethology Ecology & Evolution, Vol. 33, No. 3Direct fluorescence imaging of lignocellulosic and suberized cell walls in roots and stems29 June 2020 | AoB PLANTS, Vol. 12, No. 4Tutorial: guidance for quantitative confocal microscopy31 March 2020 | Nature Protocols, Vol. 15, No. 5F1000Research, Vol. 9Effective image visualization for publications – a workflow using open access tools and concepts18 February 2021 | F1000Research, Vol. 9Automated Macro Approach to Quantify Synapse Density in 2D Confocal Images from Fixed Immunolabeled Neural Tissue Sections21 August 2019Methods for imaging mammalian mitochondrial morphology: A prospective on MitoGraphAnalytical Biochemistry, Vol. 552Introduction and Historical Perspective31 October 2018Quantification of Image Data13 October 2017Sampling strategies to capture single-cell heterogeneity4 September 2017 | Nature Methods, Vol. 14, No. 10Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface23 February 2017 | Microscopy and Microanalysis, Vol. 23, No. 1Quality of dissolved organic matter affects planktonic but not biofilm bacterial production in streamsScience of The Total Environment, Vol. 506-507Quantification of Fluorescent Reporters in Plant Cells30 October 2014Digital pathology and image analysis in tissue biomarker researchMethods, Vol. 70, No. 1Toward an Ethical Rhetoric of the Digital Scientific Image: Learning From the Era When Science Met Photoshop9 June 2014 | Technical Communication Quarterly, Vol. 23, No. 3Intensity Calibration and Flat‐Field Correction for Fluorescence Microscopes1 April 2014 | Current Protocols in Cytometry, Vol. 68, No. 1Concepts in quantitative fluorescence microscopyEvaluating Confocal Microscopy System Performance24 August 2013Laser Scanning Confocal Microscopy: History, Applications, and Related Optical Sectioning Techniques24 August 2013Time-Profiling Fluorescent Reporters in the Arabidopsis Root26 November 2013International Test Results for Objective Lens Quality, Resolution, Spectral Accuracy and Spectral Separation for Confocal Laser Scanning Microscopes8 October 2013 | Microscopy and Microanalysis, Vol. 19, No. 6ConfocalCheck - 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