Revisão Revisado por pares

From EosFP to mIrisFP: structure‐based development of advanced photoactivatable marker proteins of the GFP‐family

2011; Wiley; Volume: 4; Issue: 6 Linguagem: Inglês

10.1002/jbio.201000122

ISSN

1864-0648

Autores

Jörg Wiedenmann, Susan Gayda, Virgile Adam, Franz Oswald, Karin Nienhaus, Dominique Bourgeois, G. Ulrich Nienhaus,

Tópico(s)

bioluminescence and chemiluminescence research

Resumo

Abstract Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non‐bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse‐chase experiments and super‐resolution imaging. In this review, we discuss the development of advanced variants, using a structure‐function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ∼400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live‐cell imaging. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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