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From proteomics to systems biology of bacterial pathogens: Approaches, tools, and applications

2007; Wiley; Volume: 7; Issue: 6 Linguagem: Inglês

10.1002/pmic.200600925

1615-9861

Uwe Plikat, Hans Voshol, Yvonne Dangendorf, Brigitte Wiedmann, Piroska Dévay, D. Müller, Urs Wirth, Joseph D. Szustakowski, Gung‐Wei Chirn, Bruno Inverardi, Xiaoling Puyang, Kara D. Brown, Heather D. Kamp, Sjouke Hoving, Alexandra Ruchti, Nathalie Brendlen, Ron Peterson, Jennifer Buco, Jan van Oostrum, Manuel C. Peitsch,

Bacterial Genetics and Biotechnology

Abstract The hallmark of a systems biology approach is the integration of computational tools with experimental data encompassing multiple classes of biomolecules across different functional levels. Equally important as the availability of reasonably comprehensive information at the gene, protein, and metabolite levels is the development of adequate analysis and visualization tools to reduce the inherent complexity to interpretable dimensions. In this paper, we describe the integration of a 2‐D gel‐based proteome map of Staphylococcus aureus Mu50 with genomic and transcriptomic information through a customized data integration and user interface built on the Ensembl genome browser. We illustrate its application and potential through the analysis of a defined system perturbation caused by a mutation in the formyltransferase gene. We envision that this software package, which we called Insieme, can support the development of novel antibiotics by allowing a systems‐based view of the bacterial response pathways.