Activation of bee venom phospholipase A 2 through a peptide‐enzyme complex
1995; Wiley; Volume: 372; Issue: 1 Linguagem: Inglês
10.1016/0014-5793(95)00964-b
ISSN1873-3468
AutoresIsmael Mingarro, Enríque Pérez‐Payá, Clemencia Pinilla, Jon R. Appel, Richard A. Houghten, Sylvie E. Blondelle,
Tópico(s)Bee Products Chemical Analysis
ResumoPhospholipase A 2 activation by membrane‐bound peptides was investigated in order to understand the role of the membrane‐induced conformation on activation, and to examine the occurrence of a peptide‐enzyme complex at the lipid/water interface. For the peptides studies, bee venom phospholipase A 2 was stimulated regardless of the membrane‐bound conformation (α‐helix, β‐sheet or random coil). Using antisera raised against melittin, we were able to demonstrate the occurrence of a calcium‐dependent complex involving the enzyme, phospholipid substrate, and peptide.
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