Produção Nacional
Revisado por pares
Purification and biological effects of Araucaria angustifolia (Araucariaceae) seed lectin
2006; Elsevier BV; Volume: 350; Issue: 4 Linguagem: Inglês
10.1016/j.bbrc.2006.09.149
ISSN1090-2104
AutoresTatiane Santi‐Gadelha, Carlos Alberto de Almeida Gadelha, Karoline S. Aragão, Cecı ́lia Carvalho de Oliveira, Mário Rogério Lima Mota, Raphaela Cardoso Gomes, Alana de Freitas Pires, Marcos Hikari Toyama, Daniela de Oliveira Toyama, Nylane Maria Nunes de Alencar, David N. Criddle, Ana Maria Sampaio Assreuy, Benildo Sousa Cavada,
Tópico(s)Biochemical Analysis and Sensing Techniques
ResumoThis paper describes the purification and characterization of a new N-acetyl-d-glucosamine-specific lectin from Araucaria angustifolia (AaL) seeds (Araucariaceae) and its anti-inflammatory and antibacterial activities. AaL was purified using a combination of affinity chromatography on a chitin column and ion exchange chromatography on Sephacel-DEAE. The pure protein has 8.0 kDa (SDS–PAGE) and specifically agglutinates rabbit erythrocytes, effect that was independent of the presence of divalent cations and was inhibited after incubation with glucose and N-acetyl-d-glucosamine. AaL showed antibacterial activity against Gram-negative and Gram-positive strains, shown by scanning electron microscopy. AaL, intravenously injected into rats, showed anti-inflammatory effect, via carbohydrate site interaction, in the models of paw edema and peritonitis. This lectin can be used as a tool for studying bacterial infections and inflammatory processes.
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