Carbon Dioxide Hydration with Liposomes Entrapping Carbonic Anhydrase
1993; Taylor & Francis; Volume: 3; Issue: 3 Linguagem: Inglês
10.3109/08982109309150746
ISSN1532-2394
AutoresMaria Cristina Annesini, Laura Di Giorgio, Luisa Di Marzio, Alessandro Finazzi‐Agrò, Annalucia Serafino, Giuseppe Mossa,
Tópico(s)Hemoglobin structure and function
ResumoLiposomes entrapping enzymes behave as small membrane bioreactors which can be used in biotechnological and biomedical applications. Furthermore, liposomes loading enzymes are being used as model to mimic cellular and subcellular organel behaviour. This work deals with the study of the kinetic behaviour of carbonic anhydrase (CA) entrapped in lipid vesicle. CA is nearly ubiquitous, being present in many tissues of different species, in cells, subcellular particles and membranes. The carbonic anhydrase entrapment in liposomes, as a function of the lipid cocktail used, was studied preliminarily. Then kinetics of carbon dioxide hydration reaction, catalysed by CA entrapped in liposomes, has been studied.
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