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Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease

2009; Elsevier BV; Volume: 5; Issue: 6 Linguagem: Inglês

10.1016/j.stem.2009.11.009

1934-5909

Krishanu Saha, Rudolf Jaenisch,

3D Printing in Biomedical Research

Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients who are classified into a disease group, thus creating "disease-specific" iPSCs. Here, we examine the challenges and assumptions in creating a disease model from a single cell of the patient. Both the kinetics of disease onset and progression as well as the spatial localization of disease in the patient's body are challenges to disease modeling. New tools in genetic modification, reprogramming, biomaterials, and animal models can be used for addressing these challenges.