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BIBTEX RIS

Molecular Characterization of Quinolone-Resistant Neisseria gonorrhoeae Isolates from Brazil

2011; American Society for Microbiology; Volume: 49; Issue: 12 Linguagem: Inglês

10.1128/jcm.01175-11

ISSN

1098-660X

Autores

Aline A. Uehara, Efigênia L. T. Amorin, Maria de Fátima Ferreira, Claudia Ferreira de Andrade, Maysa B. M. Clementino, Ivano de Filippis, Felipe Piedade Gonçalves Neves, Tatiana Castro Abreu Pinto, Lúcia Martins Teixeira, Márcia Giambiagi-deMarval, Sérgio Eduardo Longo Fracalanzza,

Tópico(s)

Syphilis Diagnosis and Treatment

Resumo

ABSTRACT Despite the rapid spread of antibiotic resistance among gonococci worldwide, limited reports are available from Brazilian locations. In the present study, 25 quinolone-resistant Neisseria gonorrhoeae (QRNG) strains isolated in Rio de Janeiro, Brazil, were characterized by phenotypic and molecular methods, including analysis of mutations in the gyrA and parC genes. They represented 16.5% of the N. gonorrhoeae isolates obtained during a survey performed from 2006 to 2010. A trend for increasing resistance to ciprofloxacin was observed in the period investigated. The most prevalent pattern of mutation observed among QRNG isolates, Ser-91 to Phe and Asp-95 to Gly in gyrA and Ser-87 to Arg in parC , was detected in 40% of the isolates exhibiting MICs ranging from 4 to >32 μg/ml. Rare types of mutations were found in the gyrA gene (Gln-102 to His [12%] and Asp-95 to Tyr [4%]) and in the parC gene (Ser-88 to Thr [4%]). The genetic relationship of the QRNG isolates, evaluated by pulsed-field gel electrophoresis, suggested that the increase in the frequencies of the QRNG isolates in Rio de Janeiro, Brazil, may have arisen as a result of simultaneous spread of two clonal groups. The results also indicate that fluoroquinolones may no longer be used as first line antibiotics for the treatment of gonorrhea in Rio de Janeiro, and that programs for antimicrobial susceptibility surveillance of N. gonorrhoeae should also be implemented in other regions of Brazil.

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