Rapid Spoligotyping of Mycobacterium tuberculosis Complex Bacteria by Use of a Microarray System with Automatic Data Processing and Assignment
2012; American Society for Microbiology; Volume: 50; Issue: 7 Linguagem: Inglês
10.1128/jcm.00442-12
ISSN1098-660X
AutoresAnke Ruettger, Johanna Nieter, Artem Skrypnyk, Ines Engelmann, Albrecht Ziegler, Irmgard Moser, Stefan Monecke, Ralf Ehricht, Konrad Sachse,
Tópico(s)Bacteriophages and microbial interactions
ResumoMembrane-based spoligotyping has been converted to DNA microarray format to qualify it for high-throughput testing. We have shown the assay's validity and suitability for direct typing from tissue and detecting new spoligotypes. Advantages of the microarray methodology include rapidity, ease of operation, automatic data processing, and affordability.
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