Artigo Acesso aberto Revisado por pares

Concentration and Diversity of Noroviruses Detected in Luxembourg Wastewaters in 2008-2009

2011; American Society for Microbiology; Volume: 77; Issue: 15 Linguagem: Inglês

10.1128/aem.00632-11

ISSN

1098-5336

Autores

Sylvain Skraber, Jérémie Langlet, Jacques R. Kremer, Joël Mossong, Sébastien De Landtsheer, Jos Even, Claude P. Muller, Lucien Hoffmann, Henry‐Michel Cauchie,

Tópico(s)

Animal Virus Infections Studies

Resumo

Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.

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