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Inheritance of the Anasazi Pattern of Partly Colored Seedcoats in Common Bean

2000; American Society for Horticultural Science; Volume: 125; Issue: 3 Linguagem: Inglês

10.21273/jashs.125.3.340

2327-9788

Mark J. Bassett, K. D. Hartel, Phil McClean,

Botanical Research and Chemistry

Inheritance of Anasazi pattern of partly colored seedcoats in common bean ( Phaseolus vulgaris L.) was studied in a genetic stock t ana B V Anasazi BC 3 5-593, whose Anasazi pattern is derived from Plant Introduction (PI) 451802. Line 5-593 is a determinate, Florida dry bean breeding line (with small black seeds) used as the recurrent parent in the development of many genetic stocks. The F 2 from the cross t ana B V Anasazi BC 3 5-593 × t z virgarcus BC 3 5-593 segregated for two nonparental phenotypic classes and was consistent with the hypothesis that a single recessive gene, with tentative symbol ana , produces the Anasazi pattern with t Z ana and a new partly colored pattern Anabip with t z ana . Thus, the Anasazi factor is not an allele at the Z locus. Analysis of 57 random F 3 progenies from the cross t ana B V Anasazi BC 3 5-593 × t z virgarcus BC 3 5-593 supported a genetic model where: 1) with t Z the Anasazi phenotype is controlled by a single recessive gene ana , i.e., genotype t Z ana , 2) the Anabip phenotype has the genotype t z ana , and 3) t Z/z ana produces a restricted Anasazi pattern. The allelism test cross t z ana Anabip BC 3 5-593 × t z l ers white BC 3 5-593 produced complementation in the F 2 , demonstrating nonallelism of Ana (actually Bip ) with the L locus. The allelism test cross t z ana Anabip BC 3 5-593 × t z bip bipunctata BC 3 5-593 failed to show complementation in F 1 and F 2 , demonstrating allelism of Ana with the Bip locus. Using bulk segregant analysis, molecular markers linked in coupling to the Ana (OM9 200 , 5.4 cM) and Bip (OJ17 700 , 6.0 cM) genes were discovered. Allelism was also suggested by the result that the same linkage distance and recombination pattern were observed when the Ana marker was used to score the bipunctata population. We propose the gene symbol bip ana for the recessive allele at the Bip locus that produces Anasazi pattern with genotype t Z bip ana and the Anabip pattern with genotype t z bip ana . Although bip ana and bip are both recessive to Bip , their interactions with the Z locus are extraordinarily different. The pattern restrictive power of bip ana expresses partly colored pattern with t Z , whereas bip requires t z to express partly colored pattern.