Portal de Periódicos da CAPES

Multiplex Immunoassay for Serological Diagnosis of Mycobacterium bovis Infection in Cattle

2008; American Society for Microbiology; Volume: 15; Issue: 12 Linguagem: Inglês

10.1128/cvi.00238-08

1556-6811

Clare Whelan, Eduard A. Shuralev, Grainne O’Keeffe, Paula Hyland, Hang Fai Kwok, Philip Snoddy, Amanda O’Brien, Marie Connolly, Padraig Quinn, Matt Groll, Todd Watterson, Sara Call, Kevin Kenny, Anthony Duignan, Mary Jo Hamilton, Bryce M. Buddle, James A. Johnston, William C. Davis, Shane A. Olwill, John Clarke,

Microbial infections and disease research

ABSTRACT Efforts to develop a better diagnostic assay for bovine tuberculosis have shown that the sensitivity and specificity of an assay can be improved by the use of two or more antigens. As reported here, we developed a multiplex chemiluminescent immunoassay that can simultaneously detect antibody activity to 25 antigens in a single well in a 96-well plate array format. The chemiluminescent signal is captured with a digital imaging system and analyzed with a macro program that tracks each serum for its pattern of antibody activity for Mycobacterium bovis antigens. The comparison of sera from 522 infected and 1,489 uninfected animals showed that a sensitivity of 93.1% and a specificity of 98.4% can be achieved with a combination of antigens. The assay system is rapid and can be automated for use in a centralized laboratory.