Portal de Periódicos da CAPES

cc SOL omics : a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli

2014; Oxford University Press; Volume: 30; Issue: 20 Linguagem: Inglês

10.1093/bioinformatics/btu420

1367-4811

Federico Agostini, Davide Cirillo, Carmen Maria Livi, Riccardo Delli Ponti, Gian Gaetano Tartaglia,

Genomics and Phylogenetic Studies

Abstract Summary: Here we introduce cc SOL omics , a webserver for large-scale calculations of protein solubility. Our method allows (i) proteome-wide predictions; (ii) identification of soluble fragments within each sequences; (iii) exhaustive single-point mutation analysis. Results: Using coil/disorder, hydrophobicity, hydrophilicity, β-sheet and α-helix propensities, we built a predictor of protein solubility. Our approach shows an accuracy of 79% on the training set (36 990 Target Track entries). Validation on three independent sets indicates that ccSOL omics discriminates soluble and insoluble proteins with an accuracy of 74% on 31 760 proteins sharing <30% sequence similarity. Availability and implementation: cc SOL omics can be freely accessed on the web at http://s.tartaglialab.com/page/ccsol_group . Documentation and tutorial are available at http://s.tartaglialab.com/static_files/shared/tutorial_ccsol_omics.html . Contact: gian.tartaglia@crg.es Supplementary information: Supplementary data are available at Bioinformatics online.