Produção Nacional
Revisado por pares
Evaluation of hematologic, blood gas, and select biochemical variables in ovine whole blood stored in CPDA ‐1 bags
2012; Wiley; Volume: 42; Issue: 1 Linguagem: Inglês
10.1111/vcp.12014
ISSN1939-165X
AutoresRejane dos Santos Sousa, Raimundo Alves Barrêto-Júnior, Isadora Karolina Freitas de Sousa, Dowglish Ferreira Chaves, Herbert Sousa Soares, Isabella Oliveira Barros, Antônio Humberto Hamad Minervino, Enrico Lippi Ortolani,
Tópico(s)Blood transfusion and management
ResumoBackground The economic consequences from mortality of sheep after blood loss can be considerable. To date there are no reports addressing hematologic, blood gas, and biochemical changes in ovine blood stored in CPDA ‐1 bags. Objectives The aim of this study was to investigate hematologic, blood gas, and biochemical alterations resulting from storage of ovine blood in CPDA ‐1 bags to establish transfusion protocols in sheep. Methods From each of 7 healthy 8‐month‐old sheep 450 mL of blood were collected into CPDA‐1 bags and stored for 35 days in at 3–6°C. Samples were taken from the bags at days 0, 7, 14, 21, and 35. Whole blood was used to assess PCV, MCV, RBC count, pH , pO 2 , pCO 2 , and concentrations of bicarbonate, sodium, and lactate. Plasma was used to measure potassium, hemoglobin, and glucose concentrations. Results The PCV remained stable throughout the storage period, while plasma hemoglobin and MCV began to increase on days 7 and 21, respectively. The RBC count began to decrease on day 21. Blood pH decreased and pCO 2 increased steadily throughout the storage period. Potassium concentration increased from 3.8 to 18.3 mmol/L on day 7 and remained high thereafter. In contrast, sodium concentration began to decrease on day 7. Conclusions The results show that ovine blood undergoes hematologic, blood gas, and biochemical changes during storage. Further studies are required to establish RBC viability in CPDA ‐1 bags after a storage period of 35 days.
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