Portal de Periódicos da CAPES

Efecto de la ticlopidina en los cambios morfológicos de cultivos endoteliales inducidos por la interacción endotoxina plaqueta

2007; National University of Colombia; Volume: 30; Issue: 2 Linguagem: Inglês

2448-4865

Nina Martínez de Lima,

Hyperglycemia and glycemic control in critically ill and hospitalized patients

La endotoxina (lipopolisacarido) induce estados trombogenicos en el shock septico. La agregacion plaquetaria inducida porlipopolisacarido es inhibida por ticlopidina. Investigamos si ticlopidina podia modificar el efecto de la interaccion lipopolisacarido - plaqueta sobre la morfologia de cultivos endoteliales. Las celulas fueron aisladas de la microvasculatura dermica de embriones de rata. Los cultivos fueron incubados con: lipopolisacarido; lipopolisacarido + plasma rico en plaquetas; lipopolisacarido + plasma rico en plaquetas de ratas pretratadas con ticlopidina; lipopolisacarido + solucion de ticlopidina; lipopolisacarido + plasma rico en plaquetas + solucion de ticlopidina; solucion de ticlopidina. Se evidencio dano celular con lipopolisacarido, que se acentuo en presencia de plasma rico en plaquetas, pero con plasma rico en plaquetas procedente de ratas pretratadas con ticlopidina, estas alteraciones no estuvieron presentes. En conclusion ticlopidina suprimio las alteraciones derivadas de la interaccion lipopolisacarido - plaqueta sobre el endotelio por lo que podria tener un potencial terapeutico en el shock septico. ABSTRACT: Endotoxin (lipopolysaccharide) contributes to the thrombogenic state, in the septic shock. Platelet aggregation induced by lipopolysaccharide is inhibited by ticlopidina. We investigated if ticlopidina could modify the effect of the lipopolysaccharide -platelets interaction, on endothelial cell morphology. The endothelial cells were isolated from rat embryos dermal microvasculature. The cultures were incubated with: lipopolysaccharide; lipopolysaccharide + platelet rich plasma; lipopolysaccharide + platelet rich plasma from rats treated with ticlopidina; lipopolysaccharide + ticlopidina solution; lipopolysaccharide + control platelet rich plasma + ticlopidina; ticlopidina solution. The results evidenced citoplasmatic alterations with lipopolysaccharide. This cellular damage was strengthen by platelet rich plasma; however, in the presence of platelet rich plasma from rats pretreated with ticlopidina, these changes did not become present. We concluded that ticlopidina suppresses some derived adverse effects of the lipopolysaccharide -platelet interaction on the endothelium, thus ticlopidina could have a therapeutic potential on septic shock.