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Beta‐carotene as a modulator of chromosomal aberrations induced in mouse bone marrow cells

1992; Wiley; Volume: 20; Issue: 3 Linguagem: Inglês

10.1002/em.2850200309

1098-2280

Daisy Maria Fávero Salvadori, Lúcia Regina Ribeiro, Marília D. M. Oliveira, Carlos Alberto de Bragança Pereira, Willy Beçak,

Carcinogens and Genotoxicity Assessment

Environmental and Molecular MutagenesisVolume 20, Issue 3 p. 206-210 Research Paper Beta-carotene as a modulator of chromosomal aberrations induced in mouse bone marrow cells Dr. Daisy M. F. Salvadori, Corresponding Author Dr. Daisy M. F. Salvadori Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilLaboratorio de Toxicologia & Genética Toxicológica, Escola de Medicina Veterinária, Universidade Federal da Bahia, Ondina, 40210, Salvador, Bahia, Brasil. The inhibitory effects of β-carotene on cyclophosphamide (CPA)-induced chromosomal aberrations in mouse bone marrow cells were investigated. Male Balb C mice, 8–10 weeks old, were treated with β-carotene (0.5, 1.0, 2.0, 5.0, 10, 25, 50, 100, and 200 mg/kg) or with corn oil (0.05 ml/10 g b.w.) by gavage for 5 consecutive days. Four hours after the last treatment with or without β-carotene, the animals were intraperitoneally injected with CPA and killed 24 hr later for cytological preparations and analysis. The results obtained show that β-carotene provides significant protection against the clastogenicity of CPA. The maximum reduction in the frequency of aberrant metaphases (26.9%) and in total number of chromosomal aberrations were observed when β-carotene was used at 50 mg/kg. Nevertheless, no direct dose-response relationship was detected, suggesting that β-carotene might act through different mechanisms at differents doses. The results obtained in animals studies have served as part of the basis for the human intervention studies now underway to determine if β-carotene does indeed function as a chemopreventive agent in human nutrition. © 1992 Wiley-Liss, IncSearch for more papers by this authorLúcia R. Ribeiro, Lúcia R. Ribeiro Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilSearch for more papers by this authorMarília D. M. Oliveira, Marília D. M. Oliveira Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilSearch for more papers by this authorCarlos A. B. Pereira, Carlos A. B. Pereira Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilSearch for more papers by this authorWilly Beçak, Willy Beçak Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilSearch for more papers by this author Dr. Daisy M. F. Salvadori, Corresponding Author Dr. Daisy M. F. Salvadori Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilLaboratorio de Toxicologia & Genética Toxicológica, Escola de Medicina Veterinária, Universidade Federal da Bahia, Ondina, 40210, Salvador, Bahia, Brasil. The inhibitory effects of β-carotene on cyclophosphamide (CPA)-induced chromosomal aberrations in mouse bone marrow cells were investigated. Male Balb C mice, 8–10 weeks old, were treated with β-carotene (0.5, 1.0, 2.0, 5.0, 10, 25, 50, 100, and 200 mg/kg) or with corn oil (0.05 ml/10 g b.w.) by gavage for 5 consecutive days. Four hours after the last treatment with or without β-carotene, the animals were intraperitoneally injected with CPA and killed 24 hr later for cytological preparations and analysis. The results obtained show that β-carotene provides significant protection against the clastogenicity of CPA. The maximum reduction in the frequency of aberrant metaphases (26.9%) and in total number of chromosomal aberrations were observed when β-carotene was used at 50 mg/kg. Nevertheless, no direct dose-response relationship was detected, suggesting that β-carotene might act through different mechanisms at differents doses. The results obtained in animals studies have served as part of the basis for the human intervention studies now underway to determine if β-carotene does indeed function as a chemopreventive agent in human nutrition. © 1992 Wiley-Liss, IncSearch for more papers by this authorLúcia R. Ribeiro, Lúcia R. Ribeiro Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilSearch for more papers by this authorMarília D. M. Oliveira, Marília D. M. Oliveira Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilSearch for more papers by this authorCarlos A. B. Pereira, Carlos A. B. Pereira Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilSearch for more papers by this authorWilly Beçak, Willy Beçak Laboratório de Toxicologia & Genética Toxicológica, Universidade Federal da Bahia, Salvador, Bahia, Brasil Instituto de Matemática e Estatistica, Universidade de São Paulo, São Paulo, Brasil Instituto Butantan, São Paulo, BrasilSearch for more papers by this author First published: 1992 https://doi.org/10.1002/em.2850200309Citations: 13AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. 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