Artigo Acesso aberto Revisado por pares

Dried Culture Spots for Xpert MTB/RIF External Quality Assessment: Results of a Phase 1 Pilot Study in South Africa

2011; American Society for Microbiology; Volume: 49; Issue: 12 Linguagem: Inglês

10.1128/jcm.05167-11

ISSN

1098-660X

Autores

Lesley Scott, Natasha Gous, Brad Cunningham, Bavesh D Kana, Olga Perovic, Linda Erasmus, Gerrit Coetzee, H. J. Koornhof, Wendy Stevens,

Tópico(s)

SARS-CoV-2 detection and testing

Resumo

Implementation of Xpert MTB/RIF requires quality assessment. A pilot program using dried culture spots (DCSs) of inactivated Mycobacterium tuberculosis is described. Of 274 DCS results received, 2.19% generated errors; the remainder yielded 100% correct Mycobacterium tuberculosis detection. The probe A cycle threshold (C(T)) variability of three DCS batches was ≤ 3.47. The study of longer-term DCS stability is ongoing.

Referência(s)