Simultaneous Estimation of Atorvastatin, Ezetimibe and Fenofibrate in Pharmaceutical Formulation by RP-LC-PDA
2010; OMICS Publishing Group; Volume: 01; Issue: 02 Linguagem: Inglês
10.4172/2153-2435.1000111
ISSN2153-2435
AutoresVishnu P. Choudhari, Anna Pratima Nikalje,
Tópico(s)Inflammatory mediators and NSAID effects
ResumoA simple, rapid and precise reversed-phase liquid chromatographic method is developed for simultaneous determination of Atorvastatin, Ezetimibe and Fenofibrate in their ternary mixture of commercial pharmaceutical preparations.This method, reported first time for a ternary mixture, uses a Kromasil C18, 250 × 4.6 mm, 5µm analytical column.Analytes were estimated by gradient elution with methanol/water at flow rate of 0.9 mL/min; the column temperature is 40°C and detector wavelength is 240 nm.The sample concentrations are measured on weight basis to avoid the internal standard.The method is validated and shown to be linear.The correlation coefficients for Atorvastatin, Ezetimibe and Fenofibrate are 0.9995, 0.9993 and 0.9996, respectively.The recovery values for Atorvastatin, Ezetimibe and Fenofibrate ranged from 99.7-101.1%,99.8-101.3%and 99.7-101.7%,respectively.The relative standard deviation for six replicates is always less than 2%.This HPLC method is successfully applied to the simultaneous quantitative analysis of the title drugs in tablets.Volume 1• Issue 2•1000111 from Ranbaxy Labs Ltd., Dewas (MP), Methanol (HPLC grade) was purchased from E. Merck (India) Ltd, Worli, Mumbai, India.Double distilled water was used throughout the experiment.Tablets were purchased from Indian market, containing FN 160 mg, AT Calcium equivalent to AT and EZ 10mg each per tablet.
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