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Micrococcus flavus sp. nov., isolated from activated sludge in a bioreactor

2007; Microbiology Society; Volume: 57; Issue: 1 Linguagem: Inglês

10.1099/ijs.0.64489-0

1466-5034

Xingyu Liu, Baojun Wang, Cheng‐Ying Jiang, Shuang‐Jiang Liu,

Bacterial Identification and Susceptibility Testing

Bacterial strain LW4 T was isolated from activated sludge of a wastewater-treatment bioreactor. Cells of strain LW4 T were Gram-positive cocci, with a diameter of 0.7–1.0 μm. Colonies produced on LB agar plates were yellow, smooth, circular and 0.5–1.5 mm in diameter. Strain LW4 T was aerobic and grew over the temperature range 26–34 °C and pH range 5–9, with optimal growth at 30.5–31.5 °C and pH 6.0–6.2. The cell-wall peptidoglycan of strain LW4 T contained amino acid residues of lysine, glutamic acid, alanine, glycine and aspartic acid. The most abundant cellular fatty acids of strain LW4 T were anteiso-C 15 : 0 (32.15 %) and iso-C 15 : 0 (31.65 %). Major respiratory quinones were MK-8(H 2 ) (57.3 %) and MK-7(H 2 ) (32.9 %). The DNA G+C content was 71.4 mol% ( T m ). 16S rRNA gene sequence analysis indicated that strain LW4 T was phylogenetically related to members of the genus Micrococcus , with similarities ranging from 96.5 to 97.3 %. Levels of DNA–DNA relatedness of strain LW4 T to Micrococcus luteus DSM 20030 T , Micrococcus lylae DSM 20315 T and Micrococcus antarcticus AS 1.2372 T were 55, 48 and 36 %, respectively. Based on these results, it is concluded that strain LW4 T represents a novel species of the genus Micrococcus , for which the name Micrococcus flavus sp. nov. is proposed. The type strain is strain LW4 T (=CGMCC 1.5361 T =JCM 14000 T ).